light cycler 480 probes master mix Search Results


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Roche 480 light cycler rt qpcr instrument
Activation of MAPK signaling pathway promoted the proliferation of PGCLCs. a Schematic diagram of the research design. b Bright field of cultures with 400 mIU LH, 25 μM MLT, or 5 μM RA for 16 days. Scale bar, 100 μm. c Flow cytometry analysis of SOX17 (FITC) and PCNA (CY3) double staining with 400 mIU LH, 25 μM MLT, or 5 μM RA. d Correlation matrix heatmap for control group, LH group, and MLT group. e The KEGG pathway enrichment of DEGs for LH vs control group and MLT vs control. f Venn diagram showed the number of enriched genes in MAPK signaling pathway for LH vs control group and MLT vs control. g Heatmap analysis of DEGs associated with cell proliferation and cell cycle for LH vs control group and MLT vs control. h The protein levels of the p‐MEK (MEK) and p‐ERK (ERK) detected by WB in culturing with LH, MLT, or RA groups for 16 days. i The expression levels of proliferation-related genes were detected by <t>RT-qPCR</t> in culturing with LH, MLT, or RA groups for 16 days. j Bright field of 10 μM U0126 culture for 16 days. Scale bar, 100 μm. k Flow cytometry analysis of SOX17 (FITC) staining with 10 μM U0126. The results are presented as mean ± SD. All the experiments were repeated at least three times. *0.01 < P < 0.05; **P < 0.01
480 Light Cycler Rt Qpcr Instrument, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TIB MOLBIOL light cycler fast start dna master hybridization probe mix
Activation of MAPK signaling pathway promoted the proliferation of PGCLCs. a Schematic diagram of the research design. b Bright field of cultures with 400 mIU LH, 25 μM MLT, or 5 μM RA for 16 days. Scale bar, 100 μm. c Flow cytometry analysis of SOX17 (FITC) and PCNA (CY3) double staining with 400 mIU LH, 25 μM MLT, or 5 μM RA. d Correlation matrix heatmap for control group, LH group, and MLT group. e The KEGG pathway enrichment of DEGs for LH vs control group and MLT vs control. f Venn diagram showed the number of enriched genes in MAPK signaling pathway for LH vs control group and MLT vs control. g Heatmap analysis of DEGs associated with cell proliferation and cell cycle for LH vs control group and MLT vs control. h The protein levels of the p‐MEK (MEK) and p‐ERK (ERK) detected by WB in culturing with LH, MLT, or RA groups for 16 days. i The expression levels of proliferation-related genes were detected by <t>RT-qPCR</t> in culturing with LH, MLT, or RA groups for 16 days. j Bright field of 10 μM U0126 culture for 16 days. Scale bar, 100 μm. k Flow cytometry analysis of SOX17 (FITC) staining with 10 μM U0126. The results are presented as mean ± SD. All the experiments were repeated at least three times. *0.01 < P < 0.05; **P < 0.01
Light Cycler Fast Start Dna Master Hybridization Probe Mix, supplied by TIB MOLBIOL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Idaho Technology Inc light cycler probe design software, version 1.0
Activation of MAPK signaling pathway promoted the proliferation of PGCLCs. a Schematic diagram of the research design. b Bright field of cultures with 400 mIU LH, 25 μM MLT, or 5 μM RA for 16 days. Scale bar, 100 μm. c Flow cytometry analysis of SOX17 (FITC) and PCNA (CY3) double staining with 400 mIU LH, 25 μM MLT, or 5 μM RA. d Correlation matrix heatmap for control group, LH group, and MLT group. e The KEGG pathway enrichment of DEGs for LH vs control group and MLT vs control. f Venn diagram showed the number of enriched genes in MAPK signaling pathway for LH vs control group and MLT vs control. g Heatmap analysis of DEGs associated with cell proliferation and cell cycle for LH vs control group and MLT vs control. h The protein levels of the p‐MEK (MEK) and p‐ERK (ERK) detected by WB in culturing with LH, MLT, or RA groups for 16 days. i The expression levels of proliferation-related genes were detected by <t>RT-qPCR</t> in culturing with LH, MLT, or RA groups for 16 days. j Bright field of 10 μM U0126 culture for 16 days. Scale bar, 100 μm. k Flow cytometry analysis of SOX17 (FITC) staining with 10 μM U0126. The results are presented as mean ± SD. All the experiments were repeated at least three times. *0.01 < P < 0.05; **P < 0.01
Light Cycler Probe Design Software, Version 1.0, supplied by Idaho Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Roche light cycler 480 instrument ii
Activation of MAPK signaling pathway promoted the proliferation of PGCLCs. a Schematic diagram of the research design. b Bright field of cultures with 400 mIU LH, 25 μM MLT, or 5 μM RA for 16 days. Scale bar, 100 μm. c Flow cytometry analysis of SOX17 (FITC) and PCNA (CY3) double staining with 400 mIU LH, 25 μM MLT, or 5 μM RA. d Correlation matrix heatmap for control group, LH group, and MLT group. e The KEGG pathway enrichment of DEGs for LH vs control group and MLT vs control. f Venn diagram showed the number of enriched genes in MAPK signaling pathway for LH vs control group and MLT vs control. g Heatmap analysis of DEGs associated with cell proliferation and cell cycle for LH vs control group and MLT vs control. h The protein levels of the p‐MEK (MEK) and p‐ERK (ERK) detected by WB in culturing with LH, MLT, or RA groups for 16 days. i The expression levels of proliferation-related genes were detected by <t>RT-qPCR</t> in culturing with LH, MLT, or RA groups for 16 days. j Bright field of 10 μM U0126 culture for 16 days. Scale bar, 100 μm. k Flow cytometry analysis of SOX17 (FITC) staining with 10 μM U0126. The results are presented as mean ± SD. All the experiments were repeated at least three times. *0.01 < P < 0.05; **P < 0.01
Light Cycler 480 Instrument Ii, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Activation of MAPK signaling pathway promoted the proliferation of PGCLCs. a Schematic diagram of the research design. b Bright field of cultures with 400 mIU LH, 25 μM MLT, or 5 μM RA for 16 days. Scale bar, 100 μm. c Flow cytometry analysis of SOX17 (FITC) and PCNA (CY3) double staining with 400 mIU LH, 25 μM MLT, or 5 μM RA. d Correlation matrix heatmap for control group, LH group, and MLT group. e The KEGG pathway enrichment of DEGs for LH vs control group and MLT vs control. f Venn diagram showed the number of enriched genes in MAPK signaling pathway for LH vs control group and MLT vs control. g Heatmap analysis of DEGs associated with cell proliferation and cell cycle for LH vs control group and MLT vs control. h The protein levels of the p‐MEK (MEK) and p‐ERK (ERK) detected by WB in culturing with LH, MLT, or RA groups for 16 days. i The expression levels of proliferation-related genes were detected by RT-qPCR in culturing with LH, MLT, or RA groups for 16 days. j Bright field of 10 μM U0126 culture for 16 days. Scale bar, 100 μm. k Flow cytometry analysis of SOX17 (FITC) staining with 10 μM U0126. The results are presented as mean ± SD. All the experiments were repeated at least three times. *0.01 < P < 0.05; **P < 0.01

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Transcriptomic landscape reveals germline potential of porcine skin-derived multipotent dermal fibroblast progenitors

doi: 10.1007/s00018-023-04869-7

Figure Lengend Snippet: Activation of MAPK signaling pathway promoted the proliferation of PGCLCs. a Schematic diagram of the research design. b Bright field of cultures with 400 mIU LH, 25 μM MLT, or 5 μM RA for 16 days. Scale bar, 100 μm. c Flow cytometry analysis of SOX17 (FITC) and PCNA (CY3) double staining with 400 mIU LH, 25 μM MLT, or 5 μM RA. d Correlation matrix heatmap for control group, LH group, and MLT group. e The KEGG pathway enrichment of DEGs for LH vs control group and MLT vs control. f Venn diagram showed the number of enriched genes in MAPK signaling pathway for LH vs control group and MLT vs control. g Heatmap analysis of DEGs associated with cell proliferation and cell cycle for LH vs control group and MLT vs control. h The protein levels of the p‐MEK (MEK) and p‐ERK (ERK) detected by WB in culturing with LH, MLT, or RA groups for 16 days. i The expression levels of proliferation-related genes were detected by RT-qPCR in culturing with LH, MLT, or RA groups for 16 days. j Bright field of 10 μM U0126 culture for 16 days. Scale bar, 100 μm. k Flow cytometry analysis of SOX17 (FITC) staining with 10 μM U0126. The results are presented as mean ± SD. All the experiments were repeated at least three times. *0.01 < P < 0.05; **P < 0.01

Article Snippet: The reaction was performed by Roche 480 Light Cycler RT-qPCR instrument (Roche, Germany) as previously described [ 36 ].

Techniques: Activation Assay, Flow Cytometry, Double Staining, Expressing, Quantitative RT-PCR, Staining